This is a disambiguation page. Alpha and Omega could refer to:
- An appellation of Christ and God in the Book of Revelation in The Bible.
- Alpha & Omega (band), a British dub/reggae duo
- Alpha and Omega (film series), a series of eight animated films produced by Crest Animation Productions
- Alpha and Omega (film), a 2010 animated comedy-drama film and a series of direct-to-DVD videos
- "Alpha and Omega", a 2016 episode of Supernatural
- A subseries of the Mercy Thompson books by Patricia Briggs, beginning with the 2007 novella Alpha & Omega
- Alpha & Omega (book), a 2003 book by Charles Seife
- Alpha Omega (board game), a 1977 board wargame published by Battleline Publications
- Alpha Omega (role-playing game), developed by Mind Storm Labs
- Pokémon Omega Ruby and Alpha Sapphire, remakes of the Pokémon Japanese role-playing games Pokémon Ruby and Sapphire
- Alpha and Omega, the doomsday bomb in Beneath the Planet of the Apes
- Alpha-Omega, a military faction in War for the Planet of the Apes
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