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Max Guevara/X5-452 (Jessica Alba)[]
- Action Girl
- Badass Biker
- Catgirl
- Child Soldiers
- Classy Cat Burglar
- Cool Big Sis: Tries to be one to Joshua (despite being younger than him).
- The Chosen One
- Deadpan Snarker
- Extraordinarily Empowered Girl
- Hero with Bad Publicity
- Meaningful Name - after guerilla leader Che Guevara
- Ms. Fanservice
- Scannable Man
- She Fu
- Sugar and Ice Personality
- Super Soldier
- Tomboyish Name
- Tyke Bomb
- Woman in Black
- You Are Number Six
Logan Cale/Eyes Only (Michael Weatherly)[]
- The Alleged Car: a Pontiac Aztek.
- Genius Cripple
- Hero with Bad Publicity
- Hollywood Hacking
- Intrepid Reporter
- Knight in Sour Armor - Lost some of his idealism over time. The prequel novel "Before the Dawn" shows that he was against killing bad guys and chewed Max's brother Seth out for doing so, but by the time of the series he's over it. Blaming himself for getting Seth killed, which he hides from Max until "After the Dark", may have something to do with it.
- Knowledge Broker
- Obfuscating Disability - After his walking problem is solved by an exoskeleton
- Perma Stubble
- Phrase Catcher
- Secret Identity - Reporter Logan Cale vs Eyes Only.
- Stoic Spectacles
- Voice of the Resistance
Original Cindy/Cynthia McEachin (Valerie Rae Miller)[]
- Dope Slap - called upon occasionally to give these to Max.
- Meaningful Rename - to Original Cindy, shedding her timid childhood persona
- Sassy Black Woman
- Shrinking Violet - her original personality.
- Third Person Person
- Twofer Token Minority - African American and lesbian
- Unfazed Everyman
Normal/Reagan Ronald (J.C. MacKenzie)[]
- Beleaguered Boss
- Deadpan Snarker
- Jerkass
- Nerd Glasses - partial example, as he wears glasses and has a less-than heroic personality.
Kendra Maibaum (Jennifer Blanc)[]
Donald Lydecker (John Savage)[]
- The Alcoholic - hasn't had a drink for 10 years when the show starts, but that doesn't last the season.
- Abusive Parent - closest thing the X5s had to a parent.
- Big Bad - in Season One
- Colonel Badass - although following the Conservation of Ninjutsu, his badassery goes up exponentially when he's by himself.
- Coup De Grace - delivers several.
- Determinator
- Enemy Mine - and he uses the associated saying
- Establishing Character Moment: Lydecker establishes himself as both a Determinator and a complete dick when he goes to an Alcoholics Anonymous meeting for the sole purpose of telling them how weak they are. You see, when he gave up drinking, he did it cold turkey under his own willpower. He didn't whine about how he had a disease and he needed help. The irony when he later falls Off the Wagon is delicious.
- Glasses Pull
- Heartbroken Badass - after his wife's pre-series death.
- Luke, I Am Your Father - discussed and lampshaded. He isn't related to Max, but did include some of his dead wife's DNA in her.
- Mercy Kill - at the end of season one, he's about to kill Max and then himself. She convinces him otherwise with a boot to the head.
- Never Found the Body
- Off the Wagon
- Offhand Backhand
- Papa Wolf - He gets extremely upset when "his kids" are endangered.
- Stoic Spectacles
- Well-Intentioned Extremist
Alec McDowell/X5-494 (Jensen Ackles)[]
- Backup Twin
- Deadpan Snarker
- Handsome Lech
- Heel Face Turn
- Lovable Rogue - called one outright by Logan; starting as a Lovable Traitor
- Meaningful Name - derived from "smart alec", actor Malcolm McMcDowell, and Malcolm's A Clockwork Orange character Alex
- Mr. Fanservice
- Pretty Boy
- Scannable Man
- Super Soldier
- Tyke Bomb
- You Are Number Six
494 provides examples of the following further tropes while posing as Simon Lehane:[]
- Becoming the Mask
- Conveniently Unverifiable Cover Story
- Dead Person Impersonation
- In Love with the Mark
- My God, What Have I Done?
- Sharp-Dressed Man
- Stoic Spectacles
- Teacher-Student Romance
Joshua (Kevin Durand)[]
- Beast Man
- Beast and Beauty - the Beast to Max's Beauty.
- And Annie's Beauty.
- Cute Little Fangs
- Expy - is rather similar in appearance and mannerisms to Vincent from the Beauty and The Beast TV series.
- Genius Bruiser: Can seem simple due to his lack of world experience, but has a high IQ.
- Gentle Giant
- The Heart
- Hulk Speak
- Reclusive Artist: For obvious reasons, he sold his paintings to the gallery via Alec.
- Third Person Person
Asha Barlow (Ashley Scott)[]
- Pair the Spares - looked like she and Alec were going this way, until they didn't
- Romantic False Lead - served as a Foil for Max and Logan
- Terrorists Without a Cause
Ames White (Martin Cummins)[]
- Big Bad - in Season Two
- Even Evil Has Loved Ones - played straight with his son, but subverted with his wife, whom he kills.
- Fantastic Racism
- Smug Snake
Zack/X5-599 (William Gregory Lee)[]
- And I Must Scream - After Manticore recaptures him, he's used as a organ donor, providing a heart to Max and liver and kidneys to others. While still alive.
- Big Brother Instinct
- Brainwashed and Crazy - in his last episode
- Child Soldiers
- Knight Templar Big Brother - unapologetically kills Vogelsang (the information broker Max hired, who was later squeezed down by Lydecker) to protect his siblings
- Neuro Vault
- Scannable Man
- Super Soldier
- Tyke Bomb
- You Are Number Six
Ben/X5-493 (Jensen Ackles)[]
- Child Soldiers
- Hunting the Most Dangerous Game
- Let Them Die Happy / Mercy Killing
- Pretty Boy
- Room Full of Crazy
- Scannable Man
- Serial Killer
- The Storyteller
- Super Soldier
- Tyke Bomb
- Used to Be a Sweet Kid
- You Are Number Six
Elizabeth Renfro/Madame X (Nana Visitor)[]
- Blondes Are Evil
- Excessive Evil Eyeshadow: A live-action version!
- Eviler Than Thou
- Expy - of the Intendent from Deep Space Nine
- Hannibal Lecture
- Have You Told Anyone Else?
- Obviously Evil
- Older Than They Look: According to Nana Visitor, Renfro used transgenic DNA to retard the aging process.
- Smug Snake
- Taking the Bullet