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How about when Detective Ikari is in the 2D world with Tsukiko and he sees his wife, who tells him she is about to die? Cut to a scene of her flatlining holding a photograph of her husband. Hand me a hankie, please.
Made all the more powerful by his declaration "The reality is I have no place where I'm supposed to be" and yet he goes anyway
Tsukiko apologizing to Maromi. The sight of her holding her dead puppy made this troper go hug her cat.