Awesome Music: Jack Sparrow and I'm On A Boat. Don't even try to deny it.
Big Lipped Alligator Moment: The entire album Bang Bang BANG DJ LARRY aside, "The Old Bang Bang BANG DJ LARRY! Saloon" quickly goes from a typical Lonely Island song to drunks constantly Bang Bang BANG DJ LARRY! interrupting each other. It's a parody of hip hop mixtapes in which way too many sound effects are played and the singers interrupt each other, thrown in with random screaming of the Bang Bang BANG DJ LAAAAAAAARRRRRRRrRrrRR...! DJ's name.
Epic Riff: The bassline for Incredibad. Also qualifies as a major Ear Worm.
Flanderization: "Dick in a Box" was a parody of cheesy '90s sex ballads. By "3-Way (The Golden Rule)", the protagonists had evolved into Disco Dans living in a caricature of the '90s.
Fountain of Memes: The main page used to have a section of nothing but memes.
Ho Yay: We'll Kill U: "Hey, Guy Number One." "Hey Guy Number Two." "I'm just doing me." "I'm doing you too." "No!"
And the entirety of "Iran so Far", towards Mahmoud Ahmadinejad.
To the singers (and the listener, if they're not into that sort of stuff) - the ending of Boombox. Elderly orgy.
In the preview to the 2011 Lonely Island album, Akiva complains about his erectile dysfunction: Yo, my dick don't work, man, that shit is soft as a pillow!
Also in "We're Back!", Akiva informs us that he suffers from Stinky Dick, wherein every time he urinates, a foul odor is emitted.
The Creep invokes this.
Motherlover is about two men who forgot Mothers Day and decide the best present they could give is to "fuck each others mothers". That's already creepy but then one of them says "while your in my mother, make me another brother" and plan on becoming each others step fathers.
Both "Classy Skits". One has Falcor raping Atreyu and the other has a man masturbating in front of his friends.