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Tim Curry briefly reprised his role as Frank N Furter for a short theater skit. Needless to say, the crowd went wild when he did the famous "Antici........(SAY IT!)....pation" line.
An in-movie example would be Columbia's truly epic verbally berating Frank for being such a heartless jerk. It doesn't end well for her but...damn!
"Jesus Christ, what a bitch! Quick, Magenta, throw the switch!"
Rocky carrying Frank up the RKO tower as anti-matter beams keep bouncing off him.
Riff Raff starting the main theme of "Timewarp." It's genuinely shocking every time when Richard O'Brien instantly changes from his "Igor" voice used in the song's introduction to full heavy metal screaming.
Eddie's only living appearance is one so you can't blame him for milking it. Coupled with Awesome Music, of course.
Frank's entrance in Sweet Transvestite, especially when he throws aside his cape to reveal his crossdressing attire.
The ending before Super Heroes. The entire castle flying off to space.
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