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There is an astonishingly funny scene around the mid-end of the second season where Gordon Cole (played by David Lynch) tries to flirt with Shelly Johnson. The running-joke of Cole's hearing problems is made fresh & funny gun, and adding the Log Lady into the mix just made it even more amazing.
Albert's speech about being an adamant pacifist.
Available here. It's one of the best moments in the show.
The moment in the season 1 finale when Andy shoots Jacques in the arm before he can shoot Truman.
The scenes where Ben Horne looks back on his childhood.
Later in season 2, when the pageant judges are discussing what they want in a Miss Twin Peaks, a very exasperated extra walks in front of them carrying a stuffed dear. It looks like he's having sex with it.
The scene where Garland Briggs slaps a cigarette of Bobby's mouth, causing it to land in Mrs. Briggs's meatloaf.
Cooper's deductive technique involving the throwing of rocks at a glass bottle.
Audrey's successful attempt to wreck her father's impending deal with the Norwegians, by informing them of Laura Palmer's murder.